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Advances in Correlative Super-resolution Light and Electron Microscopy

J.P. Gabriel

Leica – Microsysytems, Ernst-Leitz-Str. 17-37, 35578 Wetzlar, Germany

Email: Jens.Gabriel@leica-microsystems.com

BY MSSA TECH FORUM

In the past two decades, super-resolution fluorescence microscopy techniques have emerged that allow researchers to overcome the diffraction limit of light and study subcellular structures on a nanoscale. The importance of these methods have been recognized by the selection as Method of the Year 2008 (Nature Methods), and more recently by the award of the 2014 Nobel Prize in Chemistry to W. Moerner, E. Betzig and S. Hell. In close collaboration with S. Hell, Leica Microsystem has been at the forefront of the technological advances, and is offering two super-resolution microscopes: the TCS SP8 STED 3X, and the SR GSD 3D. We will discuss both technologies, as well as recent innovations on the Leica STED system which allow super-resolved imaging in 3D, and improved live-cell imaging. Today, in many disciplines such as cell biology, molecular biology, virology or neuroscience, super-resolution fluorescence microscopy is used to achieve unprecedented insights into cellular structure and dynamics. However, with these methods only fluorescently labeled proteins can be visualized, and information about non-fluorescent cellular structures is missing. Therefore, scientists are developing exciting new correlative approaches, where combining super-resolution light microscopy with electron or atomic force microscopy opens up even another new dimension for research. 

 November 06, 2015
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