PALM MicroBeam – What you see is what you get
S.L. Hendriks
Carl Zeiss (Pty) Ltd, Oak avenue 363, Ferndale, Randburg
Email: sone.hendriks@zeiss.com
The main challenges in Biomedical research is to understand the underlying function or dysfunction on molecular, cellular or tissue level. In the microscopy approach, molecules, cells or tissues are labelled with dyes or markers (for brightfield or fluorescence) to examine structures, protein localisation or even interactions. However, it is well known that the molecular analysis of RNA, DNA and proteins are beyond the capabilities of microscopes. We will demonstrate that the PALM Microbeam serves as the bridge between specimen preparation and sample selection and the functional downstream analysis. By discussing examples of DNA, RNA and protein applications as well as live cell applications for culture purification it will be shown that PALM Microbeam is an effective tool for non-contact separation of well-defined, pure, contamination-free specimens. The PALM MicroBeam system employs the proprietary LMPC method. LMPC stands for laser microdissection and pressure catapulting. This is the method that ensures contamination free specimen microdissection and capture as it is a non-contact method and works against gravity. It is unique for PALM.
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